# This file specifies additional enzymes considered for MS-GF+ # # To be loaded, this file must reside in a directory named params below the working directory # For example, create file C:\Work\params\enzymes.txt when the working directory is C:\Work # Or, on Linux, create file /home/user/work/params/enzymes.txt when the working directory is /home/user/work/ # # Format: ShortName,CleaveAt,Terminus,Description # - ShortName: A unique short name of the enzyme (e.g. Tryp). No space is allowed. # - CleaveAt: The residues cleaved by the enzyme (e.g. KR). Put "null" in case of no specificity. # - Terminus: Whether the enzyme cleaves C-terminal (C) or N-terminal (N) # - Description: Description of the enzyme # # The following enzymes are pre-configured, numbered 1 through 9 when using the -e argument at the command line # Tryp,KR,C,Trypsin # 1 # Chymotrypsin,FYWL,C,Chymotrypsin # 2 # LysC,K,C,Lys-C # 3 # LysN,K,N,Lys-N # 4 # GluC,E,C,Glu-C # 5: glutamyl endopeptidase # ArgC,R,C,Arg-C # 6 # AspN,D,N,Asp-N # 7 # aLP,null,C,alphaLP # 8 # NoCleavage,null,C,no cleavage # 9: Endogenous peptides # # If you want to redefine a pre-configured enzyme (e.g. change CleaveAt of Asp-N to "DE"), specify the enzyme again. # Specify one enzyme per line. # New enzymes will continue the numbering at 10 # # Examples: # CNBr,M,C,CNBr # AspN,DE,N,Asp-N # GluC,DE,C,Glu-C